The outbreak was confirmed in the parish of Quelfes, in the municipality of Olhão, in Faro, in a gadwall, a wild bird of the duck family.
Last week, bird flu was detected in wild birds in the parishes of Gafanha de Encarnação and Gafanha da Nazaré, in the district of Aveiro.
Earlier this month, outbreaks of infection had already been confirmed in Alcácer do Sal and Costa da Caparica.
At the time, the DGAV warned of the "persistent circulation" of the avian influenza virus and reiterated its call for adherence to biosafety measures and good production practices.
Contact between domestic and wild birds should be avoided, and hygiene procedures for facilities, equipment, and materials should be reinforced.
Transmission of the virus to humans is rare, with sporadic cases reported worldwide. However, when it does occur, infection can lead to serious clinical conditions.